Figure 2.

EPHB4-CAR-T cells demonstrated rapid and sustained antitumor activity against EPHB4-positive tumors

EPHB4-CAR-T cells, generated by our original method, were co-cultured with EPHB4-positive tumor cells at effector:target (E:T) ratios, and normalized cell index was monitored using a xCELLigence real-time cell analysis. (A) Rh30, Rh41, and RD were co-cultured with EPHB4-CAR-T cells at E:T ratios of 2:1, 1:1, and 1:2 (B and C) Serial tumor rechallenge assay; tumor cell growth was monitored via a xCELLigence real-time cell analysis (B), and the number of live tumor cells 72 h after co-culture with CD19-CAR-T cells or EPHB4-CAR-T cells in each round was measured via flow cytometry (C). Mean ± SD from 3 different experiments are shown. ∗∗p < 0.01. ∗p < 0.05. (D) Cell division of EPHB4-CAR-T cells upon repeated Rh30 cells stimulation.