Fig. 1. tNLuc-based SATiN assay for detecting α-SARS-CoV-2 antibody.
a Schematic workflow of the SATiN assay. b Scan of all probe formats/combinations in the SATiN assay using CR3022 Ab (2 µg/mL). Results are presented as a heatmap showing RLU values. Data shown here are a representative result of three independent experiments with similar results. c, d CR3022 (red) at different concentrations was tested with β9-G together with β10-S (c) or β10-S-β10 (d) probes. Human IgG (gray), a mouse monoclonal Ab (green), and rabbit polyclonal Abs (blue) were used as controls. Data shown here are a representative result of four independent experiments for each probe with similar results. e Comparison of β10-S and β10-S-β10 affinities in the SATiN system. The average Kd values of four independent experiments for each probe are presented as mean ± SD. P-value was calculated using a two-tailed t-test. Source data are available in the Source Data file.