Fig. 1. αS fibrils interact weakly with the surface of the lipid bilayer of synthetic membranes.

a PRE effects measured using MAS ssNMR for OB*/SF/LF using SUVs with DOPE:DOPS:DOPC lipid composition (molar ratio of 5:3:2) with a paramagnetic center (PC) on the bilayer surface on the hydrophilic head group (left) or in the membrane interior at carbon 16 of the lipid chain (right). ¹³C–¹³C DARR spectra measured in the presence and absence of the PC-labeled lipids are shown in blue and red, respectively. For comparison, the plots of OB* are drawn using OB* data from our previous investigation⁸. b Changes in the far-UV CD spectrum of 10 μM OB*/SF/LF and increasing concentrations of SUVs: 1 mM (red), 2 mM (blue), and 8 mM (yellow). The spectra in the absence of SUVs was subtracted in each case from that acquired in their presence. c Calcein release from SUVs (% of total intravescicular calcein—signal normalized with respect to the treatment with 1% v/v Triton X-100, see Supplementary Information for more details) upon incubation of the vesicles with the indicated αS species.