Figure 5.

Higher level of regulation by secondary vitamin D responses on transcription factors. The predicted binding of VDR, ETS1 and CEBPA¹³ to the gene loci of the primary VDR target IRF5 was confirmed by ChIP-seq assays. The binding occurred in open chromatin¹⁵ and co-located with the active enhancer mark H3K27ac¹² (a). Please note that for the VDR ChIP-seq tracks¹¹ the scale has been adapted to visualize medium-sized peaks, meaning that the summit of the strongest peak in both loci is cut. Intersects of the 1,25(OH)₂D₃ regulated genes identified from a previously published RNA-seq dataset in THP-1 cells with CEBPA knockdown¹³ with the predicted binding of CEBPA close to these genes (b). The directional network denotes which early primary VDR targeted transcription factors were predicted to bind at the gene loci of late primary VDR target transcription factors. All transcription factors shown were predicted to be bound at their gene loci by VDR (c).