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. 2021 Mar 22;80(4):336–344. doi: 10.1093/jnen/nlab018

FIGURE 3.

FIGURE 3.

Isopropyl 6-diazo-5-oxo-2-(((phenyl (pivaloyloxy) methoxy) - carbonyl) amino) hexanoate (JHU395) decreases cell proliferation. Cells treated with 1 or 2 μM JHU395 for 72 hours show decreased incorporation of BrdU by immunofluorescence. Top: 200× photomicrographs of BrdU immunofluorescence (red) of D283 medulloblastoma cells treated with JHU395 for 72 hours. DAPI counterstains nuclei blue. Bottom: BrdU immunofluorescence of D425 medulloblastoma treated with JHU395 for 72 hours. Graphs at right show quantification. Bars represent range with each replicate result represented by a solid dot. ***p < 0.001 by Student t-test (n = 5 biological replicates).