Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 5;12:609421. doi: 10.3389/fimmu.2021.609421

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Duan, Wang, Wei, Feng, Liu, He, Xu, Wang, Zhao, Lv, Long, Lin, Zhao, Fang, Jiang, Tang and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Efficient expression of CAR on lentiviral-transduced T cells and functional verification of IL-7 and CCL19. (A) Schematic representation of BCMA-hBBz CAR and BCMA-7x19 CAR. (B) CAR expressions of CAR-T cells were analyzed by flow cytometry. The numbers in scatter plots represent the percentages of positively stained cells. (C) The expression rates of BCMA CAR and BCMA-7x19 CAR. Data are means± SD obtained from seven donors. MOI = 80. P-value was calculated by two-tailed student t-test. ns, not statistically significant (P > 0.05). (D) Quantitative detection of IL-7 (left) and CCL19 (right) secretion by ELISA. (E) Number fold change of BCMA-hBBz and 7 × 19 CAR-T cells at the indicated time-points. ****P < 0.0001 by two-way ANOVA. (F) The count of migrating T cells (N = 3). ****P < 0.0001 was calculated by two-tailed student t-test. ***P < 0.001.