Figure 3.

Second round designs of junction and truncated linker guanidine biosensors. (a) Screening of 12 junction biosensors with three additional artificial stem sequences and four variable adenosine spacer lengths. (b) In vitro fluorescence response of biosensors to guanidine. Biosensors with a greater than 1.4× fluorescence increase at 1 mM guanidine are indicated in red. Nomenclature of the biosensors denotes the number of adenosines in the sequence and the identity of the P0 stem. (c) Screening of linker biosensors with seven transducer stem truncations and two variable linker lengths. (d) In vitro fluorescence response of optimized linker biosensors to guanidine. The nomenclature for linker (L) biosensors denotes the number of adenosines in the linker region and the identity of the stem truncation. Biosensors with greater than 1.2× fluorescence increase at 1 mM guanidine are indicated in red. Data shown are average with standard error of the mean for two replicates.