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. Author manuscript; available in PMC: 2021 Mar 23.
Published in final edited form as: J Immunol Methods. 2013 Nov 23;402(1-2):57–70. doi: 10.1016/j.jim.2013.11.011

Fig. 2. Effects of the Secondary Detection Antibody on GMAb ELISA Performance.

Fig. 2.

A. Use of Intact Goat-Anti-Human IgG or an F(ab’)2 Fragment as the Detection Antibody. Serum GMAb concentration measured with the GMAb ELISA in parallel using either an F(ab’)2 fragment (A2290) or intact anti-human IgG (A6029) as the detection antibody. Each symbol represents the mean of 3 determinations on the serum sample from a different autoimmune PAP patient. The median GMAb concentration (not shown) determined with the intact IgG secondary antibody was slightly higher than with the F(ab’)2 secondary antibody (128 [51 – 234] vs 118 [43 – 187] μg/mL; P< 0.001; n = 29 serum samples; Wilcoxon Signed Rank test).

B. Bland and Altman Analysis. All data shown in panel A were evaluated by Bland and Altman analysis as described in the methods.

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