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. Author manuscript; available in PMC: 2021 Mar 23.
Published in final edited form as: J Immunol Methods. 2013 Nov 23;402(1-2):57–70. doi: 10.1016/j.jim.2013.11.011

Fig. 4. Effect of Serum Aliquot Volume on GMAb ELISA Performance.

Fig. 4.

A. Effect on Serum GMAb Concentration. An initial volume of serum either 10 or 100 μl was used to measure serum GMAb concentration with the GMAb ELISA. Bars represent the mean (±SD) of 20 independent determinations using the same autoimmune patient serum. The gray line represents the mean for the data obtained using the 100 μl aliquots of serum. The difference in median (IQR) GMAb concentration for the assay performed using 10 vs 100 μl initial serum aliquots did not reach statistical significance (64.9 [52.6 – 73.4] vs 59.3 [56.0 – 64.8] μg/mL; P = 0.525, n = 20 samples; Mann-Whitney Rank Sum Test).

B. Effect on Percent Deviation of the Serum GMAb Concentration. Data from the experiment shown in panel A were used to calculate the percent deviation of the 20 determinations. The mean (±SD) percent deviation for the assay performed when using 10 μl initial serum aliquots was markedly higher than with 100 μl aliquots (17.1 ± 8.9 vs 7.1 ± 5.2; P < 0.001; n = 20 samples; Student’s t test).