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. Author manuscript; available in PMC: 2021 Mar 23.
Published in final edited form as: J Immunol Methods. 2013 Nov 23;402(1-2):57–70. doi: 10.1016/j.jim.2013.11.011

Figure 6. Specificity of the GMAb ELISA.

Figure 6.

Serum from autoimmune PAP patients (n=3) was subjected to GM-CSF affinity chromatography and the unbound fraction (flow-through) and bound fraction (after elution from the column) from each was collected as previously described (Uchida et al., 2004). The GMAb concentration was measured in serum aliquots taken before chromatography and the unbound and bound fractions. All data was normalized to the serum GMAb concentration for each patient, which was set to 100%. Bars represent the mean (±SD) amount of GMAb present in serum (hatched bar), or recovered in the Unbound (open bar) and Bound (solid bar) fractions.

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