Table 1.

Concentration Response of the Calibration Curve and Accuracy of Measuring GMAb Concentration in Human Serum Using the GMAb ELISA.

Expected concentration, ng/ml	Measured concentration, ng/ml (Mean ± SD)	Coefficient of variation (100×SD/Mean)	Standard error (%) (Mean ± SD)
Calibration curve*
50	51.9 ± 2.3	4.39	4.3 ± 3.9
25	24.4 ± 0.7	2.79	2.5 ± 2.7
12.5	12.2 ± 0.4	5.07	4.0 ± 3.5
6.25	5.8 ± 0.4	6.09	7.7 ± 5.2
3.125	3.1 ± 0.2	6.35	5.3 ± 2.5
1.5625	1.3 ± 0.1	10.58	14.8 ± 7.0
0.78125	0.8 ± 0.2	19.41	16.7 ± 12.1
Spiked samples†
10	10.1 ± 0.6	6.32	5.4 ± 2.9
20	19.0 ± 1.1	5.89	6.2 ± 4.0
40	41.8 ± 1.7	4.18	4.5 ± 4.2

^*Prepared using the autoimmune PAP-patient derived PCRS by serial dilution in sample dilution buffer.

^†Spiked samples were dilution buffers spiked with known concentration of purified GMAb.