REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
SARS-CoV-2 nucleocapsid | GeneTex | Cat#: GTX135361 RRID: AB_2887484 |
H1N1 | Abcam | Cat#ab20343, RRID:AB_445525 |
CD68 | Abcam | Cat# ab224029, clone:EPR20545 |
EpCAM | Abcam | Cat# ab213500, clone:EPR220532-222 |
alpha-SMA | Invitrogen | Cat# 53-9760-82, RRID:AB_2574461 |
KRT19 | Thermofisher | Cat# TA803863, clone: OTI6A8 |
TGFBR2 | Thermofisher | Cat# TA807912, clone: OTI3B4 |
F13A1 | Abcam | Cat# ab225018, clone: EP3372 |
CD68 | Santa Cruz | Cat# sc-20060 AF647, RRID:AB_627158 |
Biological samples | ||
Patient paraffine embedded lung tissue | UAB Tissue Biorepository Core Facility | https://sites.uab.edu/tissuebank/ |
Chemicals, peptides, and recombinant proteins | ||
Xylene | Fisher Scientific | Cat#X5P-1GAL |
100% Ethanol | Fisher Scientific | Cat#HC-800-1GL |
95% Ethanol | Fisher Scientific | Cat#HC-1100-1GL |
Tris-EDTA ph9 buffer | Abcam | Cat#ab93684 |
Bovine Serum Albumin Fraction V | Fisher Scientific | Cat#BP1605-100 |
R-Phycoerythrin lightning-link labeling kit | Novus Biologicals | Cat#703-0010 |
Alexa 647 labeling kit | Thermofisher | Cat# A20186 |
PHEM buffer | Goldbio | https://www.goldbio.com/documents/3553/PHEM+Buffer+4X+Stock+Solution.pdf |
DAPI | Biolegend | Cat#422801 |
ProLong Gold antifade mounting media | Thermofisher | Cat#P36934 |
Critical commercial assays | ||
GeoMx Cancer Transcriptome Atlas (COVID-19) | Nanostring | https://www.nanostring.com/products/geomx-digital-spatial-profiler/geomx-rna-assays/geomx-cancer-transcriptome-atlas/ |
Deposited data | ||
Transcriptomics data | This paper | https://doi.org/10.17632/n5dn4xzg7j.1 |
Software and algorithms | ||
Prism v7 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
R v3.5.2 | R project | https://www.r-project.org/ |
Microsoft Excel | Microsoft | https://www.microsoft.com/en-us/microsoft-365/p/excel/cfq7ttc0k7dx?activetab=pivot:overviewtab |
Adobe Illustrator 2020 | Adobe | https://www.adobe.com/products/illustrator.html |
GSEA | Broad Insititute | https://www.gsea-msigdb.org/gsea/index.jsp |
GeoMx DSP data center | Nanostring | https://www.nanostring.com/products/geomx-digital-spatial-profiler/geomx-data-center/ |