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. 2021 Mar 23;2(4):100245. doi: 10.1016/j.xcrm.2021.100245

Figure 1.

Figure 1

Analysis of PAC-MAN for targeting broad-spectrum RNA viruses

(A) An integrated pipeline for Cas13 crRNA analysis for virus-targeting coverage, efficiency, and specificity.

(B) Targeting pandemic/epidemic-causing pathogenic RNA virus species using PAC-MAN.

(C) Schematic of 10 RNA virus families that target different organs and systems in the human host.

(D) The minimal number of crRNAs needed to target all sequenced human-infectious strains in each of 10 RNA virus families and HIV and IAV.

(E) The minimal number of crRNAs needed to target 90% of sequenced human-infectious strains in each of 10 RNA virus families and HIV and IAV.

(F) A cumulative curve showing the predicted minimum number of crRNAs to target all human-infectious strains in 10 RNA virus families in (C).

(G) Coverage of pathogenic RNA viruses by the first 14 crRNAs in the minipool from (F).

(H) A cumulative curve showing the predicted minimum number of crRNAs to target livestock-infectious strains in 10 RNA virus families in (C). Bovine, ovine, swine, feline, and canine viruses are included in the analysis.

See also Figure S1 and Table S1.