Figure 1.
Analysis of PAC-MAN for targeting broad-spectrum RNA viruses
(A) An integrated pipeline for Cas13 crRNA analysis for virus-targeting coverage, efficiency, and specificity.
(B) Targeting pandemic/epidemic-causing pathogenic RNA virus species using PAC-MAN.
(C) Schematic of 10 RNA virus families that target different organs and systems in the human host.
(D) The minimal number of crRNAs needed to target all sequenced human-infectious strains in each of 10 RNA virus families and HIV and IAV.
(E) The minimal number of crRNAs needed to target 90% of sequenced human-infectious strains in each of 10 RNA virus families and HIV and IAV.
(F) A cumulative curve showing the predicted minimum number of crRNAs to target all human-infectious strains in 10 RNA virus families in (C).
(G) Coverage of pathogenic RNA viruses by the first 14 crRNAs in the minipool from (F).
(H) A cumulative curve showing the predicted minimum number of crRNAs to target livestock-infectious strains in 10 RNA virus families in (C). Bovine, ovine, swine, feline, and canine viruses are included in the analysis.