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. 2021 Mar 23;2(4):100245. doi: 10.1016/j.xcrm.2021.100245
REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and virus strains
Stellar chemically competent cells TaKaRa Cat# 636766
Chemicals, peptides, and recombinant proteins
Puromycin Gold Biotech Cat# P-600-1
Fetal Bovine Serum (FBS) Clontech Cat# 631367
Trypsin-EDTA solution Sigma Cat# T4049-100ML
DMEM ThermoFisher Scientific Cat# 10569-044
Mirus TranslT-LT1 Mirus Bio Cat# MIR 2306
Experimental models: Cell lines
HEK293T ATCC Cat# CRL-3216; RRID: CVCL_0063
A549 ATCC Cat# CCL-185; RRID: CVCL_0023
A549 + Cas13d (pSLQ5428) Abbott et al.2 N/A
Oligonucleotides
Primer used in qPCR: SARS-COV-F1_F: 5′-AACGGGTTTGCGGTGTAAGT-3′ Abbott et al.2 N/A
Primer used in qPCR: SARS-COV-F1_R: 5′-AA TTTAGCAAAACCAGCTACTTTATCATTGTAG-3′ Abbott et al.2 N/A
Primer used in qPCR: SARS-COV-F2_F: 5′-CAGGTGGAACCTCATCAGGAG-3′ Abbott et al.2 N/A
Primer used in qPCR: SARS-COV-F2_R: 5′-GTT ACCATCAGTAGATAAAAGTGCATTAACATTG-3′ Abbott et al.2 N/A
Primer used in qPCR: GAPDH_F: 5′-TGCACCACCAACTGCTTAGC-3′ Abbott et al.2 N/A
Primer used in qPCR: GAPDH_R:
5’-GGCATGGACTGTGGTCATGAG-3′		 Abbott et al.2 N/A
Recombinant DNA
pHR-PGK-sfGFP-SARS-COV-F1 Abbott et al.2 N/A
pHR-PGK-sfGFP-SARS-COV-F2 Abbott et al.2 N/A
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_ Cas13d-2xNLS-3xFLAG Abbott et al.2 N/A
∗pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP Abbott et al.2 N/A
∗pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroRT2A-BFP Abbott et al.2 N/A
∗Denotes generic backbone plasmid for crRNAs. Specific target sequences for crRNAs can be found in Table S2. Abbott et al.2 N/A
Software and algorithms
Cytobank Beckman Coulter N/A
FlowJo v10 https://www.flowjo.com/ N/A
Geneious 2020.0.4 https://www.geneious.com N/A
MAFFT Nakamura et al.39 https://mafft.cbrc.jp/alignment/software/
Bowtie Langmead et al.40 http://bowtie-bio.sourceforge.net/index.shtml
Bowtie 2 Langmead et al.41 http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
iTOL v5.5 Letunic and Bork18 https://itol.embl.de/
Cas13 Guide RNA Design Wessels et al.14 https://gitlab.com/sanjanalab/cas13
CRISPR-PACMAN crRNA design tool This paper https://github.com/QilabGitHub/CRISPR-PACMAN-pool-generation