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. 2021 Mar 16;23(5):367. doi: 10.3892/mmr.2021.12006

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Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — OSS-128167 aggravates myocardial inflammation and oxidative stress. (A) DAB staining (brown) was used to determine TNF-α immunoreactivity in mouse heart tissues (magnification, ×400). (B) Reactive oxygen species levels in heart tissues were detected using DHE staining (magnification, ×400). (C) Reverse transcription-quantitative PCR was used to determine the mRNA expression levels of TNF-α in myocardial tissues. (D) Levels of MDA in heart tissues. n=6-9. ***P<0.001 vs. Vehicle; ^#P<0.05, ^###P<0.001 vs. STZ-DM1. STZ, streptozotocin; STZ-DM1, STZ-induced diabetes; TNF-α, tumor necrosis factor-α; DHE, dihydroethidium; MDA, malondialdehyde.