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. 2021 Mar 16;23(5):367. doi: 10.3892/mmr.2021.12006

Figure 3.

Figure 3.

OSS-128167 aggravates HG-induced fibrosis and apoptosis of H9c2 cells. (A) Western blot analysis of COL-1 and TGF-β in H9c2 cells. H9c2 cells underwent 1 h of pre-incubation with a recommended dose of OSS-128167, followed by 36 h of 33 mM HG treatment. Proteins underwent immunoblotting, and GAPDH was used as a loading control. (B-D) H9c2 cells underwent 1 h of pre-incubation with a recommended dose of OSS-128167, followed by 12 h of 33 mM HG incubation. Reverse transcription-quantitative PCR was performed to determine the mRNA expression levels of COL-1, CTGF and TGF-β. (E and F) H9c2 cells underwent 1 h of pre-incubation with a recommended dose of OSS-128167, followed by 36 h of 33 mM HG treatment. Western blot analysis of Bcl-2 and Bax in H9c2 cells. (G) Western blot analysis of cle-PARP in H9c2 cells. GAPDH was used as the loading control. (H) H9c2 cells underwent 1 h of pre-incubation with a recommended dose of OSS-128167, followed by 12 h of 33 mM HG incubation. Caspase 3 activity assay kit was used to determine caspase 3 activity in H9c2 cells. Data from three independent experiments are presented. *P<0.05, **P<0.01, ***P<0.001 vs. Ctrl; #P<0.05, ##P<0.01, ###P<0.001 vs. HG. HG, high glucose; COL-1, collagen 1; TGF-β, transforming growth factor-β; CTGF, connective tissue growth factor; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X; cle-PARP, cleaved-poly ADP-ribose polymerase; Ctrl, control.