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. 2021 Mar 15;6(11):7374–7386. doi: 10.1021/acsomega.0c05635

Table 1. Comparision of the R3T One-Step qRT-PCR System with the Invitrogen Superscript III Platinum One-Step qRT-PCR Systema.

R3T one-step qRT-PCR system
 Invitrogen superscript III platinum one-step qRT-PCR system
sr. nos. sample N1 N2 RP expected result sr. nos. sample N1 N2 RP expected result
1 613 16.9 17.5 27.3 + positive SARS CoV-2 1 613 17.5 17.3 27.2 + positive SARS CoV-2
2 632 17.8 18.6 27.9 + positive SARS CoV-2 2 632 18.5 18.6 27.9 + positive SARS CoV-2
3 581 20.5 21.8 25.1 + positive SARS CoV-2 3 581 21.6 22.1 25.3 + positive SARS CoV-2
4 599 22.1 23.6 23.9 + positive SARS CoV-2 4 599 23.2 23.7 23.9 + positive SARS CoV-2
5 583 24.5 25.7 25.8 + positive SARS CoV-2 5 583 25.9 26.1 26.3 + positive SARS CoV-2
6 572 27.0 28.3 24.6 + positive SARS CoV-2 6 572 28.5 29.0 28.8 + positive SARS CoV-2
7 562 30.0 30.6 28.4 + positive SARS CoV-2 7 562 29.5 30.0 27.3 + positive SARS CoV-2
8 291 26.9 29.0 23.7 + positive SARS CoV-2 8 291 29.8 31.3 24.5 + positive SARS CoV-2
9 380 27.1 27.4 23.5 + positive SARS CoV-2 9 380 26.8 27.1 24.4 + positive SARS CoV-2
10 550 32.8 33.1 26.9 + positive SARS CoV-2 10 550 33.0 33.9 26.3 + positive SARS CoV-2
11 552 32.6 35.4 30.7 + positive SARS CoV-2 11 552 34.2 34.7 30.2 + positive SARS CoV-2
12 554 33.9 35.0 31.9 + positive SARS CoV-2 12 554 33.2 33.4 28.6 + positive SARS CoV-2
13 585 35.5 38.4 24.1 + positive SARS CoV-2 13 585 35.4 37.6 23.3 + positive SARS CoV-2
14 600 35.3 38.3 26.2 + positive SARS CoV-2 14 600 35.9 37.2 26.3 + positive SARS CoV-2
15 601 34.6 36.7 23.4 + positive SARS CoV-2 15 601 35.2 36.1 23.0 + positive SARS CoV-2
16 745 34.0 37.0 28.1 + positive SARS CoV-2 16 745 35.3 36.7 28.1 + positive SARS CoV-2
17 748 31.3 33.4 27.0 + positive SARS CoV-2 17 748 32.1 34.1 26.9 + positive SARS CoV-2
18 749 34.3 36.1 32.9 + positive SARS CoV-2 18 749 34.1 34.7 32.5 + positive SARS CoV-2
19 751 35.0 37.2 29.6 + positive SARS CoV-2 19 751 34.9 36.1 28.6 + positive SARS CoV-2
20 750 34.5 38.3 37.6 + positive SARS CoV-2 20 750 35.2 36.8 36.7 + positive SARS CoV-2
21 411 – – 27.5 – negative SARS CoV-2 21 411 36.9 – 29.1 – negative SARS CoV-2
22 429 – – 26.9 – negative SARS CoV-2 22 429 39.3 40.5 27.5 – negative SARS CoV-2
23 440 – – 25.1 – negative SARS CoV-2 23 440 37.6 – 25.5 – negative SARS CoV-2
24 + 24.8 25.1 24.8 – positive control 24 + 23.6 23.6 24.2 – positive control
25 NTC – – – – not detected 25 NTC – – – – not detected
a

N1 and N2: N-gene: RP: RNaseP; NTC: no template.