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Target gene of miR-613. (A) Potential binding sites between miR-613 and GJA1. Dual-luciferase reporter assay in (B) H1299 and (C) A549 cells showed that miR-613 targeted GJA1. Following overexpression of miR-613 in H1299 and A549 cells, the (D) mRNA and (E) protein expression levels of GJA1 were significantly decreased. *P<0.05. miR-613, microRNA-613; NC, negative control; WT, wild-type; MUT, mutant; GJA1, gap junction α-1 protein; 3′-UTR, 3′-untranslated region.
