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. 2021 Mar 18;23(5):385. doi: 10.3892/mmr.2021.12024

Figure 6.

Figure 6.

miR-613-induced chemosensitivity of lung cancer cells to CDDP is partially reversed by GJA1. (A) mRNA expression levels of GJA1 were upregulated in lung cancer tissues. (B) Pearson correlation analysis revealed that miR-613 was negatively correlated with GJA1 mRNA expression (R2=0.2408; P=0.0109) in NSCLC. (C) Expression levels of GJA1 in lung cancer tissues were significantly increased. (D and E) Transfection efficiency of GJA1 overexpression plasmid in H1299 and A549 cells. GJA1 overexpression inhibited (F) cell proliferation and (G) migration. (H) Overexpression of miR-613 in H1299 and A549 enhanced the inhibitory effect of CDDP on the proliferation of lung cancer cells, whereas overexpression of GJA1 partially reversed this effect. (I) Overexpression of miR-613 in H1299 and A549 cells enhanced the inhibitory effect of CDDP on the migration of lung cancer cells, whereas overexpression of GJA1 partially reversed this effect. Scale bar, 100 µm. *P<0.05 vs. NC or as indicated. miR-613, microRNA-613; NC, negative control; CDDP, cisplatin; OD, optical density; GJA1, gap junction α-1 protein.