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. Author manuscript; available in PMC: 2021 Mar 23.
Published in final edited form as: Cell Rep. 2021 Mar 2;34(9):108797. doi: 10.1016/j.celrep.2021.108797

Figure 4. Resolution of HO conflicts by Type II topoisomerases promotes the formation of R-loops.

Figure 4.

(A and B) DRIP-seq (A) and DnaC (B) ChIP-seq profiles of cells lacking RNase HIII harboring either an HO (blue, strain HM2043) or CD (strain HM2044) lacZ engineered conflict treated or untreated with novobiocin. The bottom panel of both DRIP-seq profiles is the RNase-H-treated control. Normalized signal is the read depth of IP/input normalized to the total number of reads.

(C) Survival of cells lacking RNase HIII harboring either an HO (strain HM2043) or CD (strain HM2044) lacZ engineered conflict treated or untreated with novobiocin.

See also Figures S4 and S5 and Tables S4–S6.