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. 2021 Mar 23;12:11. doi: 10.1186/s13100-021-00239-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — A dendrogram of IS6 family members.: A dendrogram of IS6 family members. The figure shows 11 major clades. The surrounding colored circles and the insert indicate the clades identified by [40]. The insert shows the correspondence between the clades from Harmer and Hall and those defined here. Clades: A: composed almost entirely of archea; Ai: (n = 12) is composed of diverse Halobacterial species (Halohasta, Haloferax, Natrinema, Natrialba, Halogeometricum, Natronomonas, Natronococcus, and Haloarcula); Aii: (n = 12) is composed uniquely of Halobacterial Euryarchaeota; Aiii: (n = 5) is composed entirely of Halobacterial Euryarchaeota (Haloarcula, Halomicrobium, Natronomonas, Natronobacterium, Natrinema); Aiv: (n = 9) which includes both Euryarchaeota and Crenarchaeota; b: (n = 16) Actinobacteria, Alpha-, Beta-, and Gamma-proteobacteria; c: (n = 14) Alphaproteobacteria: Rhizobiaceae and Methylobacteriaceae); d: (n = 24) (Alpha-, Beta-, and Gamma-proteobacteria, Firmicutes, Cyanobacteria, Acidobacteria and Bacteroidetes); e: (n = 23) is composed mainly of IS from Lactococcus, a single Leuconostoc and other bacilli (Lysteria, Enterococcus); f: (n = 11) largely Staphylococci with 2 B. thuringiensis; g: (n = 10) is heterogenous (Alpha proteobacteria: Methylobacterium, Paracoccus, Roseovarius, Rhizobium, Bradyrhizobium; Deinococci and Halobacteria); h: (n = 5) composed entirely of Firmicutes (Natranaerobius, Clostridium and Thermoanaerobacter); i: (n = 3) is composed of Halanaerobia and Thermoanaerobacter. TnpA protein sequences retrieved from ISfinder curated data set were aligned with MAFFT 7.309, and their best-fit evolutionary models were predicted with ProTest 3.2.4. A maximum likelihood tree was reconstructed with RaxML 8.2.9 using a bootstrap value of 1000. The final tree was visualized in FigTree 1.4.4 (http://tree.bio.ed.ac.uk/software/figtree) and edited with Inkscape 0.92.4 (http://www.inkscape.org)