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. 2021 Mar 23;12:11. doi: 10.1186/s13100-021-00239-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Two Models for TU formation from the Pseudo-compound Transposon Tn4325B. Symbols are as in the previous figures. The small filled circle within one of the internal IS flanks (white arrow) indicates the additional GG dinucleotide carried by Tn4325B. Both use an intramolecular replicative transposition pathway in a cis configuration. In the top panel, the wildtype IS uses the flank of the mutated IS as a target. This would generate a TU with a single IS and both internal flanking sequences and an excisant with two tandem IS copies separated by a mutant flank. In the lower panel, the TU carries two tandem IS copies and the excisant