Fig. 6.

Interaction of GhARF2b and GhHOX3. a Diagram of protein domains in ARF protein. b GhARF2b and GhARF2b middle region can interact with GhHOX3 in Y2H assay. The figure shows yeast grown on SD/−Leu-Trp-His with 5 mM 3AT (3-amino-1,2,4-triazole). c GhHOX3 and GhARF2b are interchangeably fused to the carboxyl- and amino-terminal of firefly luciferase (LUC, LUCc and LUCn), transiently co-expressed, and LUCc or LUCn was co-expressed with each other or with each unfused target protein as the control. Fluorescence signal intensities represent their binding activities