Fig. 2.

NIN protein is nuclear located in Medicago nodules. Pink nodules formed on nin‐1 roots transformed with ProNIN:NIN‐GFP (a) and ProNIN:GFP‐NIN (f) constructs at 6 wk post inoculation (wpi). Confocal images show that both NIN‐GFP (b–e) and GFP‐NIN (g–j) are located in the nucleus of cells in the meristem (c, h), infection zone (b–d, g–i), fixation zone (d, i) and vasculature (e, j). Transgenic roots were identified by DsRED fluorescent (red colour). Arrowheads indicate amyloplast deposition. Arrows indicate nucleus. M, meristem; IF, infection zone; FX, fixation zone; V, vasculature. Bars: (a, f) 2 mm; (b, g) 250 µm; (c–e, h–j) 40 µm.