Table 3.

Susceptibility of L. gasserie HHuMIN D culture supernatant antimicrobial activity to various hydrolytic enzymes

Enzyme	Activity
Control (no-enzyme)	+++
Proteinase K	−
Trypsin	−
α-Chymotrypsin	−
Pepsin	+++
α-Amylase	+++

Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, and Porphyromonas catoniae were used as indicators. Degree of growth inhibition by OD (600 nm): “+++”, value similar to control; “++”, value weaker than control; “−”, no growth. Culture supernatant was mixed with enzyme solutions at a final concentration of 1 mg/ml