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. 2021 Mar 23;20:75. doi: 10.1186/s12934-021-01563-w

Table 7.

The number of experimental bacteria and pathogens bound to KB cells in adhesion ability, protection assays and displacement assays

Strains No. of CFU recovered a (× 105) Adhesion inhibitory rate (%)
Positive Control L. gasseri HHuMIN D 4.41 ± 1.4
F. nucleatum KCOM1001 18.35 ± 4.0
S. mutans KCTC3065 2.57 ± 0.1
Protection assaysb (L. gasseri HHuMIN D → F. nucleatum KCOM1001) L. gasseri HHuMIN D 1.64 ± 0.2** 63
(L. gasseri HHuMIN D → F. nucleatum KCOM1001) F. nucleatum KCOM1001 0.05 ± 0.0*** 100
(L. gasseri HHuMIN D → S. mutans KCTC3065) L. gasseri HHuMIN D 1.27 ± 0.6** 71
(L. gasseri HHuMIN D → S. mutans KCTC3065) S. mutans KCTC3065 0.25 ± 0.2**** 90
Displacement assaysc (F. nucleatum KCOM1001 → L. gasseri HHuMIN D) L. gasseri HHuMIN D 1.32 ± 0.4** 70
(F. nucleatum KCOM1001 → L. gasseri HHuMIN D) F. nucleatum KCOM1001 2.10 ± 0.8*** 89
(S. mutans KCTC3065 → L. gasseri HHuMIN D) L. gasseri HHuMIN D 1.18 ± 0.3** 73
(S. mutans KCTC3065 → L. gasseri HHuMIN D) S. mutans KCTC3065 0.27 ± 0.1**** 90

aValues represent the means ± the standard deviations for triplicate samples of lysates from the infection of 105 KB cells by 108 bacteria. Compared with the positive control group: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (n = 3)

bProtection assays are a method of investigating how harmful bacteria are inhibited from attaching by selected bacteria which are already attached

cDisplacement assays are a method of investigating the degree of attachment by selected bacteria after harmful bacteria are already attached