Table 7.
Strains | No. of CFU recovered a (× 105) | Adhesion inhibitory rate (%) | |
---|---|---|---|
Positive Control | L. gasseri HHuMIN D | 4.41 ± 1.4 | |
F. nucleatum KCOM1001 | 18.35 ± 4.0 | ||
S. mutans KCTC3065 | 2.57 ± 0.1 | ||
Protection assaysb | (L. gasseri HHuMIN D → F. nucleatum KCOM1001) L. gasseri HHuMIN D | 1.64 ± 0.2** | 63 |
(L. gasseri HHuMIN D → F. nucleatum KCOM1001) F. nucleatum KCOM1001 | 0.05 ± 0.0*** | 100 | |
(L. gasseri HHuMIN D → S. mutans KCTC3065) L. gasseri HHuMIN D | 1.27 ± 0.6** | 71 | |
(L. gasseri HHuMIN D → S. mutans KCTC3065) S. mutans KCTC3065 | 0.25 ± 0.2**** | 90 | |
Displacement assaysc | (F. nucleatum KCOM1001 → L. gasseri HHuMIN D) L. gasseri HHuMIN D | 1.32 ± 0.4** | 70 |
(F. nucleatum KCOM1001 → L. gasseri HHuMIN D) F. nucleatum KCOM1001 | 2.10 ± 0.8*** | 89 | |
(S. mutans KCTC3065 → L. gasseri HHuMIN D) L. gasseri HHuMIN D | 1.18 ± 0.3** | 73 | |
(S. mutans KCTC3065 → L. gasseri HHuMIN D) S. mutans KCTC3065 | 0.27 ± 0.1**** | 90 |
aValues represent the means ± the standard deviations for triplicate samples of lysates from the infection of 105 KB cells by 108 bacteria. Compared with the positive control group: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (n = 3)
bProtection assays are a method of investigating how harmful bacteria are inhibited from attaching by selected bacteria which are already attached
cDisplacement assays are a method of investigating the degree of attachment by selected bacteria after harmful bacteria are already attached