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. 2021 Mar 23;54:11. doi: 10.1186/s40659-021-00335-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The effects of high circRSF1 expression on cell proliferation, apoptosis and inflammatory of ox-LDL-treated HUVECs was reversed by miR-135b-5p overexpression. a The expression of miR-135b-5p was measured in control, ox-LDL, ox-LDL + vector, ox-LDL + circRSF1, ox-LDL + circRSF1 + miR-NC and ox-LDL + circRSF1 + miR-135b-5p groups using qRT-PCR, the measurements of the following indicators are grouped in this experiment. b MTT assay was applied to measure cell proliferation. c Flow cytometry was used to detect cell apoptosis. d Western blot assay detected the protein expression of Bcl-2, Bax and cleaved-caspase-3. e–h ELISA was used to assess the level of IL-1β, IL-6, TNF-α and IL-8 in control, ox-LDL, ox-LDL + vector, ox-LDL + circRSF1, ox-LDL + circRSF1 + miR-NC and ox-LDL + circRSF1 + miR-135b-5p groups. i Western blot assay detected the protein expression of VCAM1, ICAM1 and E-selectin. ‘ + ’ was added, ‘−’ was blank. Each experiment was performed in triplicate *p < 0.05