Fig. 5.

HDAC1 was a target mRNA of miR-135b-5p in HUVECs. a The predicted binding sites of miR-135b-5p to the HDAC1 sequences using circbank. b Luciferase reporter assay determined that miR-135b-5p was a target miRNA of circRSF1 in HUVECs. c, d qRT-PCR and western blot were used to detect the mRNA and protein expression of HDAC1 in miR-NC, miR-135b-5p, anti-miR-NC and anti-miR-135b-5p groups in HUVECs. e, f qRT-PCR and western blot were used to detect HDAC1 mRNA and protein expression in control and ox-LDL groups in HUVECs. g, h qRT-PCR and western blot were used to detect HDAC1 mRNA and protein expression in vector, circRSF1, circRSF1 + miR-NC, circRSF1 + miR-135b-5p groups in HUVECs. Each experiment was performed in triplicate *p < 0.05