Fig. 4.

Cell type-specific mitochondrial morphology in dopaminergic neurons derived from TH-GFP iPSCs. a Representative SEM images of GFP-negative non-dopaminergic neurons (top) and GFP-positive dopaminergic neurons (bottom) derived from control TH-GFP iPSCs. Scale bar, 1 µm. b Representative SEM images of GFP-negative non-dopaminergic neurons (top) and GFP-positive dopaminergic neurons (bottom) derived from PRKN-mutated TH-GFP iPSCs. “PRKN” represents PRKN-mutated patient. Scale bar, 1 µm. c Quantitative analysis of the mitochondrial area from the SEM images of GFP-negative and -positive cells derived from control and PRKN-mutated TH-GFP iPSCs. “PRKN” represents PRKN-mutated patient. Mitochondrial area (control GFP-nega; n = 199, control GFP-posi; n = 198, PRKN GFP-nega; n = 215, PRKN GFP-posi; n = 200) was measured using ImageJ. Values are shown as the mean ± SD. Statistical significance was evaluated using the two-way ANOVA with Šidák’s multiple comparisons test. **P < 0.01, ****P < 0.0001. There was no significant difference in the mitochondrial area between control and PRKN-mutated GFP-positive cells (P = 0.298). d Quantitative analysis of the mitochondrial major axis from the SEM images of GFP-negative and -positive cells derived from control and PRKN-mutated TH-GFP iPSCs. “PRKN” represents PRKN-mutated patient. The mitochondrial major axis (control GFP-nega; n = 199, control GFP-posi; n = 198, PRKN GFP-nega; n = 215, PRKN GFP-posi; n = 200) was measured using ImageJ. Values are shown as the mean ± SD. Statistical significance was evaluated using the two-way ANOVA with Šidák’s multiple comparisons test. ****P < 0.0001. There was no significant difference in the mitochondrial major axis between the GFP-positive and -negative PRKN-mutated cells (P = 0.1651). There was no significant difference in the mitochondrial major axis between control and PRKN-mutated GFP-positive cells (P = 0.7795)