Table 1.

Sequence of primers for RT-PCR and long PCR

Target Gene	Primer Sequence	Size (bp)	Tm (°C)
Mfn1	Forward: 5′-GGGAAGACCAAATCGACAGA-3′	152	57
	Reverse: 5′-CAAAACAGACAGGCGACAAA-3′		57
Mfn2	Forward: 5′-GAGAGGCGATTTGAGGAGTG-3′	165	58
	Reverse: 5′-CTCTTCCCGCATTTCAAGAC-3′		56
Drp1	Forward: 5′-GCCCGTGGATGATAAAAGTG-3′	215	56
	Reverse: 5′-TGGCGGTCAAGATGTCAATA-3′		56
PGC-1α	Forward: 5′-GGACGAATACCGCAGAGAGT-3′	201	59
	Reverse: 5′-CCATCATCCCGCAGATTTAC-3′		56
Nrf1	Forward: 5′-AAACCGAACACATGGCTACC-3′	168	58
	Reverse: 5′-CTGCCGTGGAGTTGAGTATG-3’		58
Tfam	Forward: 5′-TCACCTCAAGGGAAATTGAAG-3′	241	55
	Reverse: 5′-CCCAATCCCAATGACAACTC-3’		56
Long Fragment	Forward:5′-AAAATCCCCGCAAACAATGACCACCC-3’	13,400	72
	Reverse: 5′-GGCAATTAAGAGTGGGATGGAGCCAA-3’		72
Shrot Fragment	Forward: 5′-CCTCCCATTCATTATCGCCGCCCTGC-3’	235	60
	Reverse: 5′-GTCTGGGTCTCCTAGTAGGTCTGGGAA-3’		60
Bax	Forward: 5′-GCGATGAACTGGACAACAAC-3′	200	57
	Reverse: 5′-GATCAGCTCGGGCACTTTAG-3’		58
Bcl-2	Forward: 5′-CGAGTGGGATACTGGAGATGA-3’	236	58
	Reverse: 5′- GACGGTAGCGACGAGAGAAG-3’		59
Caspase-3	Forward: 5′-CCCATCACAATCTCACGGTAT-3’	195	57
	Reverse: 5′-GGACGGAAACAGAACGAACA-3’		58
Caspase-9	Forward: 5′-GCCTCTGCTTTGTCATGGAG-3’	181	56
	Reverse: 5′-AGCATGAGGTTCTCCAGCTT-3’		56
PI3K	Forward: 5′-TCACCTCCCTGATTGGCTAC-3’	220	58
	Reverse: 5′-CCACGATGGATGACAATGAA-3′		55
Akt	Forward: 5′-CGAGTCCCCACTCAACAACT-3′	231	59
	Reverse: 5′-GGTGAACCTGACCGGAAGTC-3’		60
Bad	Forward: 5′-GAGCTGACGTACAGCGTTGA-3’	153	60.39
	Forward: 5′-CCTGAGGGCTGTCCAGTAAC-3’		59.75
PARP	Forward: 5′-AAGCCTGGCACTAAGTCGAA-3’	164	59.31
	Forward: 5′-ATAGAGTAGGCGGCCTGGAT-3’		59.88
Cyc-c	Forward: 5′-GGACAGCCCCGATTTAAGTA-3’	121	57
	Forward: 5′-TCAATAGGTTTGAGGCGACAC-3’		58
GAPDH	Forward: 5′- AGGTCGGTGTGAACGGATTTG −3’	20	58
	Reverse: 5′- GGGGTCGTTGATGGCAACA-3’		58