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. 2021 Mar 5;8(2):ENEURO.0199-20.2021. doi: 10.1523/ENEURO.0199-20.2021

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Copyright © 2021 Rapp et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 2. — Schematic of EBC behavioral apparatus. Upper left. Video display of mouse with open eye during baseline. Lower left. Display depicting mouse with closed eyelids during a CR. Middle. Depiction of head-fixed mouse atop the freely rotating cylinder. Camera for visualizing mouse during conditioning task on left of cylinder frame. Speakers behind mouse are used to deliver tone CS. Blunted needle delivers aversive airpuff US to eye. Custom headbolt implanted on the mouse’s head connects to amplifier to receive EMG signal (depicted on the right).