Table 1.
Sample type | Dilution level | No. of replicates | Number of pixels |
---|---|---|---|
Positive controls | No dilution | 12 | 3,189 |
Positive controls | 1:2 | 12 | 2,780 |
Positive controls | 1:10 | 12 | 2,428 |
Positive controls | 1:50 | 12 | 1,804 |
Positive controls | 1:100 | 12 | 2,523 |
Positive controls | 1:500 | 12 | 2,183 |
Positive pixels subtotal | 14,907 | ||
Background (area around dots) | NA | NA | 44,993 |
Buffer alone | No dilution | 13 | 2,426 |
Negative controls | No dilution | 11 | 2,984 |
Negative pixels subtotal | 50,403 | ||
Total | 65,310 |
NA, Not Applicable.