Figure 4. Dysregulation of endothelial cell mRNA transcripts when nonclassical monocytes fail to patrol.

A) Scheme of bone marrow transplantation for integrin knockout chimeras with E2 bone marrow donors at a 50:50 ratio. B-M) Endothelial cells were digested out of lungs harvested from E2:WT and E2:K-KO BMT mice, with and without i.v. injection of 5×10⁵ B16F10 cells, and endothelial cells were then sorted and purified for mRNA. B-M) qPCR for inflammatory cytokines, integrin ligands, and other signaling cytokines were run in duplicate with Hprt used as a normalization control. n = 3–4 mice per group. N) Heat map of transcript signatures for each group of mixed BMT mice with and without B16F10 cancer cells injected. Each gene expression value was rescaled to reflect the range of expression for each individual gene. Blue = Homeostasis, Pink = Metastasis. ns = not significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.