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. 2021 Jan 29;599(6):1855–1883. doi: 10.1113/JP281044

Figure 12. Expression of Nalcn transcript in the mouse adrenal gland, with a specific localisation in CCs.

Figure 12

A, real‐time PCR determination of transcripts encoding NALCN and the ancillary proteins Unc79, Unc80 and NLF‐1 in the mouse adrenal tissue (n = 4 mice). Mouse brain was used as a positive tissue expressing NALCN and the liver as a negative tissue. Note that whole adrenal glands (including both cortex and medulla) were used. B, double in situ hybridization used for the co‐detection of Nalcn and dopamine beta hydroxylase (Dbh) transcripts in the mouse adrenal gland. Ba, specific detection of Nalcn mRNA with the antisense probe showing that Nalcn expression is restricted to the adrenomedullary tissue (M, specifically labelled by Dbh mRNA, which encodes a biosynthetic catecholamine enzyme) and excluded from the cortex (C). Specific sense probe showed no labelling. Bb, co‐localisation of Nalcn (pseudo green fluorescent labelling) and Dbh (pseudo red fluorescent labelling) arguing for a specific expression of NALCN in CCs.