Figure 5.

Mucosal CD8 and CD4 T cell memory in vaccinated mice. Cohorts of mice were vaccinated twice, as described in Figure 2 . At 100 days after booster vaccination, to stain vascular cells, mice were injected intravenously with PE-labeled anti-CD4.2 antibody. Cells from BAL and lungs were stained with D^b/NP366 tetramers or I-A^b/NP311 tetramers along with antibodies for the indicated cell surface molecules. Percentages and total numbers of NP366-specific CD8 T cells or NP311-specific CD4 T cells in BAL or lungs (A, B, respectively). Graphs in panels (A, B) also show total numbers of CD103⁺CD69⁺ and nonvascular NP366- or NP311-specific T cells in lungs. Plots in (C) are gated on NP311-specific T cells and graphs show percentages of the indicated subsets among tetramer-binding CD4 T cells. Data are representative of three independent experiments. *, **, and *** indicate significance at P<0.1, 0.01, and 0.001, respectively.