Figure 2.

Silencing RhoV inhibited A549 and PC9 cell proliferation and tumor formation in vivo. (A, B) qRT-PCR and western blotting analysis of esiRhoV decreased the mRNA and protein expression of RhoV in A549 and PC9 cells. (C, D) CCK8 and cell counting analysis of silencing RhoV effect of cell viability and growth speed. (E, F) Colony formation was detected by crystal violet staining after the transient transfection of esiRhoV for 14 days. (Compared with match control, *p < 0.05, **p < 0.01). (G) Western blotting analysis of the stable knockdown and rescue effect by overexpression of RhoV in A549 and PC9 cells. (H, I) CCK8 analysis of the growth curve in stable knockdown RhoV and rescue by overexpression RhoV in A549 and PC9 cell lines. (J, K) A549 (shScramble group and shRhoV group) cells were injected subcutaneously to NOD-SCID mice at 5 × 10⁶ per mouse. Tumor volumes were measured and recorded every week (n = 5) (J). At 8 weeks after injection, the mice were sacrificed, and tumors were photographed or weighed (K). (L) IF analysis of Ki-67 expression in shScramble and shRhoV tumors. (Compared with match control, *p < 0.05, **p < 0.01; compared with shRhoV, ^##p < 0.01).