Figure 4.

Only Group 3 and Group 4 MB cell lines show laminar growth patterns on HA gels, while SHH cell lines are characterized by nodular growth that can only be altered by vitronectin. MB cell lines representing the different subgroups were seeded on top of pure HA hydrogels or on hydrogels supplemented with either laminin or vitronectin. Growth patterns were monitored over time (number in the bottom right corner equals day of growth). All Group 3 and Group 4 cell lines grow as relatively even laminar monolayers on all three different hydrogels and can cover the whole area within a few weeks. In contrast, SHH cell lines struggle to adhere on pure HA and form a single central spheroid‐like structure instead over time. On laminin‐supplemented HA hydrogels, these cells adhere and form multiple nodules over time. In contrast, the vitronectin‐supplemented hydrogels provide the only surface where SHH MB cell lines grow as a thin laminar monolayer (scale bar = 100 μm). The complete time course for all cell lines can be found in supplementary material, Figures S8–S10.