Table 2.
Bacterial growth inhibition by meropenem in the presence of cephalosporin prodrug 6 against clinical isolates of MBL‐producing Gram‐negative bacteria.
|
Strain |
MBL |
MIC [μg mL−1][a] |
FIC[b] |
|
|---|---|---|---|---|
|
|
|
meropenem |
+6 |
|
|
K. pneumoniae (strain JS022) |
NDM‐1 |
16 |
1 (16) |
≤0.188 |
|
K. pneumoniae (strain N11‐2218) |
NDM‐1 |
32 |
4 (8) |
≤0.250 |
|
K. pneumoniae (strain RC0048) |
VIM‐1 |
64 |
4 (16) |
≤0.188 |
|
K. pneumoniae (strain RC0021) |
VIM‐1 |
16 |
0.5 (32) |
≤0.156 |
|
K. pneumoniae (strain NRZ‐293) |
VIM‐2 |
4 |
0.13 (32) |
≤0.156 |
|
K. pneumoniae (strain JS265) |
IMP‐28 |
4 |
0.13 (32) |
≤0.156 |
|
E. coli (strain RC0089) |
NDM‐1 |
32 |
1 (32) |
≤0.156 |
|
E. coli (strain 2018–2015) |
NDM‐1 |
8 |
0.5 (16) |
≤0.188 |
|
E. coli (strain 1322) |
VIM‐2 |
4 |
0.25 (16) |
≤0.188 |
|
E. coli (strain 2018–014) |
IMP‐4 |
8 |
0.063 (128) |
≤0.133 |
|
P. aeruginosa (strain NRZ‐8418) |
NDM‐1 |
64 |
32 (2) |
NS[c] |
|
P. aeruginosa (strain 581) |
VIM‐2 |
16 |
8 (2) |
NS[c] |
|
P. aeruginosa (strain 2018–009) |
IMP‐13 |
32 |
32 (1) |
NS[c] |
[a] Minimum inhibitory concentration (MIC) values given in μg mL−1 with the fold improvement in brackets. Compound 6 administered at 64 μg mL−1. Values are the mean of at least three experiments. [b] FIC=(MIC of meropenem in combination/MIC of meropenem alone)+(MIC of inhibitor in combination/MIC of inhibitor alone). FIC values below 0.5 indicate a synergistic effect. [c] NS=no synergy.