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Determination of key enzymatic parameters for SARS-CoV-2 3CL-Pro: (A) fluorescence signal versus time for enzyme concentrations from 0 to 0.25 μM (substrate concentration = 15 μM); (B) replotting the t = 15 min data against enzyme concentration; (C) determination of Km and Vmax in the presence and absence of DTT ([SARS-CoV-2 3CL-Pro] = 60 nM). Reaction rate calculations used increase in signal between 0 and 2 min.
