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[Preprint]. 2021 Mar 17:rs.3.rs-279341. [Version 1] doi: 10.21203/rs.3.rs-279341/v1

Figure 1. MISTRG6 humanized mice that transiently express hACE2 can be infected with SARS-CoV-2.

Figure 1.

A. Schematic of experimental design.

B. Viral RNA (quantification of N gene) C. Viral titers measured by PFU in homogenized lung tissue at 2, 4, 7 14, 28 days post infection (dpi) in B6 control or reconstituted or unengrafted MISTRG6 mice expressing or lacking human ACE2. N=2–24. Means with SD of at least 2 independent experiments are presented.

D. Weight change during the course of infection plotted as percent change compared with original weight measured just before inoculation with SARS-Cov2. N=6–37. Means with SD of at least three independent experiments are presented.

E. Weight change of individual mice reconstituted MISTRG6-hACE2 mice on 2,4,7,10,14,21 and 28dpi. N=6–37.

F. Histopathology by H&E staining of infected (2, 4, 7, 14 and 28dpi) or uninfected lungs. N=4–8 per time point. Representatives of at least three independent experiments are presented.

G. Trichrome staining of infected lungs at 28dpi. Arrows indicate areas with Collagen deposition. N=4–8 per time point.

Human ACE2 was delivered by AAV to lungs of wild type or reconstituted adult MISTRG6 mice. Mice were then infected (or left uninfected) intranasally with SARS-CoV-2 and weighed on 0, 2, 4, 7, 14, 21 and 28 dpi.