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[Preprint]. 2021 Mar 17:rs.3.rs-309746. [Version 1] doi: 10.21203/rs.3.rs-309746/v1

Fig.5: Phenotypic characterization of SARS-CoV-2-specific CD4 and CD8 T cells from convalescent HIV negative and HIV positive subjects.

Fig.5:

a Representative flow plots and b pie charts representing proportion of antigen-specific CD4 T cell with a CD45RA−/CCR7+ central memory (CM), CD45RA+/CCR7+ naïve, CD45RA+/CCR7− terminally differentiated effector memory (TEMRA) and CD45RA−/CCR7− effector memory (EM) phenotype from HIV negative (HIV−, n=12) and HIV positive individuals (HIV+, n=11) against SARS-CoV-2 Spike, M, N, CMV pp65 and HIV gag. c Representative flow plots and d pie charts representing proportion of CD45RA−/CCR7+ central memory (CM), CD45RA+/CCR7+ naïve, CD45RA+/CCR7− terminally differentiated effector memory (TEMRA) and CD45RA−/CCR7− effector memory (EM) antigen-specific CD8 T cell subsets against SARS-CoV-2 Spike, M, N, CMV pp65 and HIV gag. e Representative flow plots from an HIV negative donor (HIV−) and an HIV positive donor (HIV+) showing expression of CD154 and IFN-g production from PD1+ and PD1− SARS-CoV-2-specific CD4 T cells and paired analysis of responses in HIV negative (HIV−, n=12) and HIV positive (HIV+, n=11) individuals. f Correlation between frequency of (PD-1+CD154+IFN-g+ SARS-CoV-2-specific CD4 T cells and DPSO in both groups. Significance determined by Wilcoxon matched-pairs signed rank test, *p<0.05, **p<0.01, ***p < 0.001. The non-parametric Spearman test was used for correlation analysis; p values for individual correlation analysis within groups, HIV−, HIV+, or combined correlation analysis (black) are presented.