Table 1.
Primers for detection of enteric viruses in commercial turkeys with hatchery disease
| Virus | Primer | Nucleotide sequence (5'-3') | Target gene | Reference |
|---|---|---|---|---|
| Infectious bronchitis virus | IBV5 F | GCTTTTGAGCCTAGCGTT | S1 gene | [20] |
| IBV5 R | GCCATGTTGTCACTGTCTATT | |||
| Probe | FAM-CACCACCAGAACCTGTCACCTC-BHQ1 | |||
| Chicken astrovirus | CAstV F | GCYGCTGCTGAAGAWATA CAG | Polymerase | [21] |
| CAstV R | CATCCCTCTACCAGATTTTCT GAA A | |||
| Probe | 6-FAM-CAG AAG TCG GGC CC-MGB | |||
| Avian nephritis virus | ANV F | GTA AAC CAC TGG YTG GCT GAC T | Polymerase | [21] |
| ANV R | TAC TCG CCG TGG CCT CG | |||
| Probe | 6-FAM-CAG CAA CTG ACT TTC-MGB | |||
| Turkey astrovirus 1 | TAstV-1 F | AGCTYATGMGGTTCTTTCTTCTYG | Polymerase | [22] |
| TAstV-1 R | GATGGTGGGTAGCCTATTGTGTTC | |||
| Turkey astrovirus 2 | TAstV-2 F | TGGACCGACCCRRTTTTYACCA | Polymerase | [22] |
| TAstV-2 R | GGCCCGACYTCAGGMAGTTGT | |||
| Avian rotavirus | AvRV(ROT) F | GGG CGT GCG GAA AGA TGG AGA AC | NSP4 | [22] |
| AvRV(ROT) R | GGG GTT GGG GTA CCA GGG ATT AA | |||
| Avian reovirus | ARV (Reo) P1F | AGT ATT TGT GAG TAC GAT TG | Sigma C | [18] |
| ARV (Reo) P4R | GGC GCC ACA CCT TAG GT | |||
| Chicken astrovirus | CAstV (pre cap) F | TAG AGG GAT GGA CCG AAA TAT AGC AGC | ORF 2 (capsid) | [23] |
| CAstV (post cap) R | TGC AGC TGT ACC CTC GAT CCTA | |||
| Fowl adenovirus | FAdV Hex(A) F | CAA RTT CAG RCA GAC GGT | Hexon | [19] |
| FAdV Hex(B) R | TAG TGA TGM CGS GAC ATC AT |