Table 1.
Differentially expressed proteins (DEPs) identified by iTRAQ and 2DE in TKR/UKR blood plasma samples compared to HCs
| List of DEPs identified by 2DE | List of DEPs identified by both techniques | |||||||
|---|---|---|---|---|---|---|---|---|
| % Cov (95) | Accession | Name | Peptides (95%) | Spots, n | Name/accession | Peptides (95%) | Name/accession | |
| 1 | 56 | sp|P02768|ALBU_HUMAN | Serum albumin OS=Homo sapiens OX=9606 GN=ALB PE=1 SV=2 | 181 | 1 | Haptoglobin/ sp|P00738|HPT_HUMAN | 24 | Serum albumin/ sp|P02768|ALBU_HUMAN |
| 2 | 46.7 | sp|P02787|TRFE_HUMAN | Serotransferrin OS=Homo sapiens OX=9606 GN=TF PE=1 SV=3 | 147 | 2 | Transthyretin/ tr|A6XGL1|A6XGL1_HUMAN | 209 | Haptoglobin/ tr|J3QR68|J3QR68_HUMAN |
| 3 | 47.4 | sp|P01023|A2MG_HUMAN | α2 macroglobulin OS=Homo sapiens OX=9606 GN=A2M PE=1 SV=3 | 140 | 3 | Transthyretin/ tr|A6XGL1|A6XGL1_HUMAN | 27 | |
| 4 | 59.8 | sp|P01009|A1AT_HUMAN | α1 antitrypsin OS=Homo sapiens OX=9606 GN=SERPINA1 PE=1 SV=3 | 138 | 4 | Haptoglobin/ sp|P00738|HPT_HUMAN | 72 | |
| 5 | 58.7 | tr|J3QR68|J3QR68_HUMAN | Hbα (fragment) OS=Homo sapiens OX=9606 GN=HP PE=1 SV=1 | 87 | 5 | Haptoglobin/ sp|P00738|HPT_HUMAN | 42 | |
| 6 | 81.7 | sp|P69905|HBA_HUMAN | Hbα OS=Homo sapiens OX=9606 GN=HBA1 PE=1 SV=2 | 69 | 6 | Epididymis secretory sperm binding protein/ tr|E9KL23|E9KL23_HUMAN | 3 | |
| 7 | 37.6 | sp|P00450|CERU_HUMAN | Ceruloplasmin OS=Homo sapiens OX=9606 GN=CP PE=1 SV=1 | 51 | 7 | cDNA FLJ78413/ tr|A8K9P0|A8K9P0_HUMAN | 50 | |
| 8 | 19.2 | sp|P01024|CO3_HUMAN | Complement C3 OS=Homo sapiens OX=9606 GN=C3 PE=1 SV=2 | 32 | 8 | sp|P02768|ALBU_HUMAN | 100 | |
| 9 | 65.3 | sp|P68871|HBB_HUMAN | Hbβ OS=Homo sapiens OX=9606 GN=HBB PE=1 SV=2 | 32 | 9 | cDNA FLJ78413/ tr|A8K9P0|A8K9P0_HUMAN | 131 | |
| 10 | 43.1 | sp|P02647|APOA1_HUMAN | ApoA1 OS=Homo sapiens OX=9606 GN=APOA1 PE=1 SV=1 | 30 | 10 | Epididymis secretory sperm binding protein/ tr|E9KL23|E9KL23_HUMAN | 3 | |
| 11 | 33.8 | sp|P02790|HEMO_HUMAN | Hemopexin OS=Homo sapiens OX=9606 GN=HPX PE=1 SV=2 | 28 | 11 | Haptoglobin/ sp|P00738|HPT_HUMAN | 178 | |
| 12 | 26.9 | sp|P04217|A1BG_HUMAN | α1B glycoprotein OS=Homo sapiens OX=9606 GN=A1BG PE=1 SV=4 | 27 | 12 | HP Protein/ tr|Q6NSB4|Q6NSB4_HUMAN | 212 | |
| 13 | 58.5 | sp|P02042|HBD_HUMAN | Hbδ OS=Homo sapiens OX=9606 GN=HBD PE=1 SV=2 | 26 | 13 | Haptoglobin/ tr|J3QR68|J3QR68_HUMAN | 196 | |
| 14 | 57.8 | sp|P02766|TTHY_HUMAN | Transthyretin OS=Homo sapiens OX=9606 GN=TTR PE=1 SV=1 | 18 | 14 | HP Protein/ tr|Q0VAC5|Q0VAC5_HUMAN | 92 | |
| 15 | 32.8 | sp|P00739|HPTR_HUMAN | Haptoglobin-related protein OS=Homo sapiens OX=9606 GN=HPR PE=2 SV=2 | 17 | 15 | Haptoglobin/ tr|J3QR68|J3QR68_HUMAN | 75 | |
| 16 | 25.4 | sp|P02763|A1AG1_HUMAN | α1-acid glycoprotein 1 OS=Homo sapiens OX=9606 GN=ORM1 PE=1 SV=1 | 15 | ||||
| 17 | 14.7 | tr|C9JV77|C9JV77_HUMAN | α2-HS glycoprotein OS=Homo sapiens OX=9606 GN=AHSG PE=1 SV=1 | 14 | ||||
| 18 | 20.4 | tr|D6RF35|D6RF35_HUMAN | Vitamin D–binding protein OS=Homo sapiens OX=9606 GN=GC PE=1 SV=1 | 12 | ||||
| 19 | 17.6 | tr|A0A286YEY1|A0A286YEY1_HUMAN | IgG1 (fragment) OS=Homo sapiens OX=9606 GN=IGHA1 PE=1 SV=1 | 12 | ||||
| 20 | 22 | sp|P06727|APOA4_HUMAN | ApoA4 OS=Homo sapiens OX=9606 GN=APOA4 PE=1 SV=3 | 9 | ||||
| 21 | 20.1 | tr|A0A0G2JPA8|A0A0G2JPA8_HUMAN | α2 antiplasmin OS=Homo sapiens OX=9606 GN=SERPINF2 PE=1 SV=1 | 9 | ||||
| 22 | 11 | sp|P43652|AFAM_HUMAN | Afamin OS=Homo sapiens OX=9606 GN=AFM PE=1 SV=1 | 9 | ||||
| 23 | 22.9 | sp|P19652|A1AG2_HUMAN | α1-acid glycoprotein 2 OS=Homo sapiens OX=9606 GN=ORM2 PE=1 SV=2 | 9 | ||||
| 24 | 36.8 | tr|V9GYM3|V9GYM3_HUMAN | ApoA2 OS=Homo sapiens OX=9606 GN=APOA2 PE=1 SV=1 | 8 | ||||
| 25 | 6.7 | tr|B7ZKJ8|B7ZKJ8_HUMAN | ITIH4 protein OS=Homo sapiens OX=9606 GN=ITIH4 PE=1 SV=1 | 8 | ||||
| 26 | 11.8 | sp|P01019|ANGT_HUMAN | Angiotensinogen OS=Homo sapiens OX=9606 GN=AGT PE=1 SV=1 | 6 | ||||
| 27 | 9.9 | tr|A0A286YEY4|A0A286YEY4_HUMAN | Ig heavy constant γ2 (fragment) OS=Homo sapiens OX=9606 GN=IGHG2 PE=1 SV=1 | 6 | ||||
| 28 | 13.3 | tr|A0A1B0GUU9|A0A1B0GUU9_HUMAN | Ig heavy constant μ (fragment) OS=Homo sapiens OX=9606 GN=IGHM PE=1 SV=1 | 5 | ||||
| 29 | 3.8 | sp|P02671|FIBA_HUMAN | Fibrinogen α chain OS=Homo sapiens OX=9606 GN=FGA PE=1 SV=2 | 4 | ||||
| 30 | 7.8 | sp|P02750|A2GL_HUMAN | Leucine-rich α2 glycoprotein OS=Homo sapiens OX=9606 GN=LRG1 PE=1 SV=2 | 4 | ||||
| 31 | 4.6 | sp|P00488|F13A_HUMAN | Coagulation factor XIII A chain OS=Homo sapiens OX=9606 GN=F13A1 PE=1 SV=4 | 4 | ||||
| 32 | 14.7 | sp|P27169|PON1_HUMAN | Serum paraoxonase/arylesterase 1 OS=Homo sapiens OX=9606 GN=PON1 PE=1 SV=3 | 4 | ||||
| 33 | 8.2 | sp|P01008|ANT3_HUMAN | Antithrombin III OS=Homo sapiens OX=9606 GN=SERPINC1 PE=1 SV=1 | 3 | ||||
| 34 | 21.5 | sp|P00915|CAH1_HUMAN | Carbonic anhydrase 1 OS=Homo sapiens OX=9606 GN=CA1 PE=1 SV=2 | 3 | ||||
| 35 | 0.6 | sp|P04114|APOB_HUMAN | ApoB100 OS=Homo sapiens OX=9606 GN=APOB PE=1 SV=2 | 2 | ||||
| 36 | 5 | sp|P22792|CPN2_HUMAN | Carboxypeptidase N2 OS=Homo sapiens OX=9606 GN=CPN2 PE=1 SV=3 | 2 | ||||
| 37 | 7.1 | sp|P02760|AMBP_HUMAN | Protein AMBP OS=Homo sapiens OX=9606 GN=AMBP PE=1 SV=1 | 2 | ||||