Figure 4. Hfq-RNase E interaction contributes to the recycling of Hfq from the mRNA-associated form to the mRNA-free form.

(a) Diffusion coefficients are plotted for WT, Q8A, and Y25D Hfq-mMaple3 in the rne131 and rneΔ14 backgrounds under NT and Rif conditions. (b) mRNA-associated fraction of Hfq, for the same cases in (a). p-values are reported between WT rne and rne mutants under Rif condition. Error bars in all plots represent the s.d. from two experimental replicates, with each data set containing ~5,000 trajectories (for D value calculation) or ~20,000 trajectories (for mRNA-associated fraction calculation) from ~100 cells. All fitting results are reported in Supplementary file 1.