Table 3. The potency of HC-070 on various human TRPC5 (hTRPC5) constructs.

TRPC5 constructs	HC-070
	LogIC50*	Hill slope	IC50 (nM)
hTRPC5 WT	2.31 ± 0.10	−1.52	204.3
Mutations in HC-070-binding pocket
C525A	2.16 ± 0.15	−1.03	145.8
R557A	2.52 ± 0.07	−1.45	329.7
F569A	2.86 ± 0.15	−1.21	727.4
L572A	2.74 ± 0.17	−1.58	552.7
Q573A	3.10 ± 0.61	−1.75	>1000
F599A	2.67 ± 0.29	−1.09	467.9
F599N	3.57 ± 1.55	−0.86	>1000
T603A	2.68 ± 0.16	−1.20	472.7
T607A	2.43 ± 0.12	−3.13	268.4

^* Data were expressed as logIC₅₀ ± SEM, n = 3.