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. 2021 Mar 15;2021:5271291. doi: 10.1155/2021/5271291

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Copyright © 2021 Biyong Deng et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

LncRNA RBM5-AS1 promoted Os cells proliferation in vitro. (a) Stable lncRNA RBM5-AS1 knockdown in MG63 and 143B cells was confirmed by RT-PCR. (b) CCK8 assays showed the effect of RBM5-AS1 on the proliferation of MG63 and 143B cells. (c, d) Colony formation assays showed a significantly lower proliferative rate in both cell lines following RBM5-AS1 knockdown. (e, f) Flow cytometry was used to explore whether RBM5-AS1 promoted Os cell proliferation. (g) Western blotting was used to detect the protein level of cyclin B1, cyclin D1, CDK4, and CDK6 after RBM5-AS1 inhibition.