Figure 13.

Silencing BRD4 Down-Regulates the Transcription of PLK4 and PDGFRα

Rat adventitial fibroblasts were transfected with a scrambled or BRD-specific siRNA for 48 hours and collected for (A) Western blot and (B) qRT-PCR assays. (A) Comparison of the effects of silencing BRD2. BRD3, or BRD4 on PLK4 protein levels. Shown are representative blots from 1 of 2 similar repeated experiments. (B) Silencing BRD4 reduces the protein levels of PDGFRα, PLK4, PLK1, and αSMA. Quantification: densitometry normalized to β-actin, mean ± SEM, n = 5 independent experiments, representative blots from one of which are shown on the right. Student t-test: ∗∗p < 0.01; ∗∗∗p < 0.001. (C, D) Effects of BRD4 or BRD2 silencing on mRNA levels of PDGFRα, PLK4, and PLK1 (qRT-PCR data). Quantification: mean ± SD of triplicates; shown is 1 of 2 similar experiments. Student t-test: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. BRD, bromodomain protein; other abbreviations as in Figures 1, 4, and 11.