Figure 17.

Perivascular Administration of PLK4 Inhibitor Reduces αSMA in the Adventitia of Injured Arteries

A. Representative immunofluorescence images for αSMA. Elastin autofluorescence was used to distinguish the external elastic lamina (indicated by open arrowheads). The strong αSMA signal in the media layer, where smooth muscle cells (high αSMA expression) reside, presents a positive control validating αSMA immunostaining. Scale bar: 100 μm. (B) Quantification. The immunofluorescence intensity in the adventitial layer (measured with the use of ImageJ above a threshold) was normalized to the number of DAPI-stained corresponding adventitial cells in each image field. The data from different sections were pooled to generate the mean for each animal. The means from all animals in each group were then averaged to produce the final mean ± SEM (n = 3 animals per group). Student t-test: ∗p < 0.05.