Figure 3.

PLK1 Inhibition Blocks PDGF-AA–Stimulated Fibroblast Cell-Type Transition

Experiments were performed as described in Figure 1 except that the PLK1-selective inhibitor GSK461364 (GSK4) was used for pretreatment before AA stimulation. (A) Western blots of αSMA and vimentin. (B) Morphologic comparison. Cells were (or were not) stimulated by AA for 24 h without or with pretreatment (1 μmol/l GSK4). (C) Proliferation. CellTiter-Glo assay was performed after 72 h stimulation (by AA or solvent) without or with pretreatment with GSK4 at increasing concentrations. (D) Migration (scratch assay). Cells were (or not) stimulated by AA for 24 h without or with pretreatment (0.1 or 1 μmol/l GSK4). Mean ± SEM, n ≥3 experiments, 1-way ANOVA/Bonferroni test: #p < 0.05; ##p < 0.01; ###p < 0.001. ∗p < 0.05; ∗∗∗p < 0.001 compared with vehicle control without AA (C) or with AA (A, D). Abbreviations as in Figure 1.